Last Modified: Friday, 11-Oct-2019 08:30:19 EDT

Cryo-electron microscopy is a developing alternative to X-ray crystallography for 3-D structure determination. The applications below are some of the more commonly-used programs. See the Core system administrator if other programs are required.

Note that the Core is not involved in the actual sample preparation or data collection process. There are several Yale facilities offering cryo-EM services.

The first step when using Core resources is making your data locally accessible. It is usually possible to attach a portable drive to Core workstations and using

mount /local/disk1

etc., to access the contents. Remember to use

umount /local/disk1

etc, to safely remove the drive.

For the Science Hill Cryo-EM facility, the current data directory is directly accessible from Core workstations in the directory

/srv/basstalos



In the case of the School of Medicine EM facility, the current data directory for the T12 is directly accessible from Core workstations by going to the directory

cd /srv/T12_User_Images

For data access to the West Campus Cryo-EM facility, contact the center's staff.

Note that such shared folders' contents are generally read-only, so you will probably want to transfer your data to a local external drive or to /srv/frames.

Several of the programs below either run on the command line, or have portions that can optionally do so. In such cases, it might be worth looking into using the batch queue system, especially the "cryo" queue that runs on the multi-GPU system.